NHS-activated Sepharose® 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling. For applications that require operation at high pH, note that the amide bond formed when using the companion product NHS-activated Sepharose 4 Fast Flow is stable up to pH 13 for normal use. CNBr-activated Sepharose 4 Fast Flow is supplied freeze-dried in the presence of additives.

Zhivie oboi na rabochij stol windows 7 vesna. Crayon shin chan manga raw download. Solutions & buffers Step 1 Activation buffer. Dilute 4 μl HCl in 50 ml water for a final concentration of 1 mM Coupling buffer. Component Final concentration Stock Amount NaHCO 3, pH 8.3 100 mM 1 M 100 ml NaCl 500 mM 5 M 100 ml Add water to 1 l Step 3 Quenching buffer. Dilute 5 ml of 1 M Tris–HCl, pH 8.0 in 45 ml water for a final concentration of 100 Mm Step 4 High pH wash buffer. Component Final concentration Stock Amount Tris–HCl, pH 8.0 100 mM 1 M 25 ml NaCl 500 mM 5 M 25 ml Add water to 250 ml Low pH wash buffer. Component Final concentration Stock Amount NaOAc, pH 4.0 100 mM 1 M 25 ml NaCl 500 mM 5 M 25 ml Add water to 250 ml Storage buffer (PBS, pH 7.4) Component Final concentration Stock Amount NaCl 137 mM 5 M 1.37 ml KCl 2.7 mM 4 M 33.8 μl NaH 2PO 4 10 mM 0.5 M 1 ml K 2HPO 4 2 mM 0.5 M 0.2 ml Add water to 50 ml.

NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. • NHS pre-activated resin for coupling of small amino-containing proteins and peptides in process-scale applications • High level of pre-activation gives a high degree of substitution of the selected ligand • NHS coupling method results in chemically stable ligand attachment • BioProcess resin supported for industrial applications.